Detección de los tipos virales 16,18, 31 y 33 de papillomavirus humano en displasias severas y carcinoma in situ de cuello uterino

Guillermo Rodríguez; Isabel Fernández; Enrique Barrios; Carlos Sanguinetti; Rafael Alonso; Noemí Maedo; Juan Vasallo

Authors

Guillermo Rodríguez Comisión Honoraria de Lucha contra el Cáncer, Programa de Prevención del Cáncer de Cuello Uterino, Coordinador. Universidad de la República, Facultad de Medicina, Clínica Ginecotocológica, Ex Profesor Adjunto
Isabel Fernández Hospital Gustavo Saint Bois, Laboratorio de Anatomía Patológica, Jefe. Universidad de la República, Facultad de Medicina, Anatomía Patológica, Ex Profesora Adjunta
Enrique Barrios Universidad de la República, Facultad de Medicina, Métodos Cuantitativos, Profesor Titular
Carlos Sanguinetti Universidad de la República, Facultad de Ciencias, Magíster en Biología
Rafael Alonso Universidad de la República, Facultad de Medicina, Métodos Cuantitativos, Profesor Agregado
Noemí Maedo Hospital Policial, Laboratorio de Anatomía Patológica, Jefa. Universidad de la República, Facultad de Medicina, Anatomía Patológica, Ex Profesora Adjunta
Juan Vasallo Comisión Honoraria de Lucha contra el Cáncer, Programa de Vigilancia Epidemiológica, Ex Coordinador

Keywords:

PAPILLOMAVIRUS INFECTIONS, UTERINE CERVICAL DYSPLASIA, CARCINOMA IN SITU, UTERINE CERVICAL NEOPLASMS

Abstract

Introduction: severe dysplasia and carcinoma-in-situ (CIN) is actually the first grade of uterine cervical cancer. HPV infection constitutes an important etiological factor for the origin of High-grade Squamous Intraepithelial Lesion (H-SIL) and uterine cervical epidermoid cancer.
Objective: to determine the prevalence of HPV types that are described as more frequent in other regions, from a sample of Uruguayan women carriers of severe dysplasia and uterine cervix carcinoma in situ, with diagnosis confirmed by the histological study of surgical conization pieces.
Method: we selected a sample of surgical conization pieces from women who were carriers of severe dysplasia and intraepithelial epidermoid cancer of the uterine cervix in Montevideo and Canelones. Pieces were fixed in formol and then embedded in paraffin.
Results: we identified the presence of HPV deoxiribonucelic acid (DNA) in 39 out of 49 patients (80%). The most common type of virus found was HPV 16 in 23 of cones (47%), followed by HPV 33, found in seven cases (14%). We stand out the absence of HPV 18 in this sample.
Conclusion: we proved the usual association between HPV infections and precursor lesions to uterine cervical cancer. Among the four types of HPV studied and described as more common in other regions, we identified HPV 16 as the most common, the same as it happens in other parts of the world.

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