Comparación de las tinciones con hematoxilina y eosina versus azul de toluidina en la evaluación de los carcinomas cutáneos durante la cirugía micrográfica de Mohs

Erika Lutz; Virginia Rivero; Nelson Turra; Camila Otero; Cecilia  Fernández; Alicia  González; Verónica Lezué; Analía  Guerra; Julio Magliano; Carlos Bazzano

doi:10.29193/RMU.38.1.8

Authors

Erika Lutz Hospital de Clínicas Dr. Manuel Quintela. Médica dermatóloga
Virginia Rivero Hospital de Clínicas Dr. Manuel Quintela. Médica posgrado de dermatología
Nelson Turra Chile, Hospital Puerto Montt Dr. Eduardo Schütz Schroeder. Médico dermatólogo
Camila Otero Hospital de Clínicas Dr. Manuel Quintela. Médica dermatóloga
Cecilia Fernández Hospital de Clínicas Dr. Manuel Quintela. Médica dermatóloga
Alicia González Hospital de Clínicas Dr. Manuel Quintela, Técnica anatomía patológica
Verónica Lezué Hospital de Clínicas Dr. Manuel Quintela, Técnica anatomía patológica
Analía Guerra Hospital de Clínicas Dr. Manuel Quintela, Cátedra de Dermatología, Asistente. Médica dermatóloga y dermopatóloga
Julio Magliano Hospital de Clínicas Dr. Manuel Quintela, Unidad de Cirugía Dermatológica, Jefe. Cátedra de Dermatología, Profesor Adjunto. Médico dermatólogo, cirujano de Mohs
Carlos Bazzano Hospital de Clínicas, Dr. Manuel Quintela, Cátedra de Dermatología, Profesor Agregado. Médico dermatólogo, Encargado del Servicio

DOI:

https://doi.org/10.29193/RMU.38.1.8

Keywords:

MOHS MICROGRAPHIC SURGERY, SKIN CANCER, COLORING AGENTS, HEMATOXYLIN, EOSINE, TOLUIDINE BLUE, BASAL CELL CARCINOMA, SQUAMOUS CELL CARCINOMA

Abstract

Introduction: Mohs micrographic surgery is a specialized surgical technique used to treat nonmelanoma carcinoma. Histopathology plays a vital role in the diagnosis of this condition, and the choice staining method is controversial.
Objective: to compare results in the use of hematoxylin and eosin (H&E) versus Toluidine blue (TB) staining during surgery.
Method: observational, descriptive and transversal study conducted from November, 2017 until May, 2018 of the slides used during surgeries in the selected period. Slides were analysed by the Mohs surgeon, 3 residents and a dermopathologist to evaluate the results of both staining methods, in consideration of cell features and stromal elements.
Results: 23 tumors were analysed (16 Basal Cell carcinomas and 7 Squamous Cell Carcinoma). Microscopic observation of slides prepared with Toluidine blue and hematoxylin and eosin stains did not show significant global differences between both groups, except in terms of a few characteristics, in particular with hematoxylin and eosin stains.
Conclusions: this was the first study in Uruguay to evaluate the effectiveness of both staining methods during Mohs micrographic surgery, and it concluded that both Toluidine blue and hematoxylin and eosin stains are very good techniques in evaluating skin-cancer.

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