Genotype characterization of 80 strains of Mycobacterium in Uruguay
Keywords:
MYCOBACTERIUM, MYCOBACTERIUM TUBERCULOSIS, MYCOBACTERIOSIS, GENOTYPEAbstract
Traditional methods to determine phenotype identification for mycobacterium are longer as compared with cellular procedures (4 to 6 weeks and 36 to 72 hours respectively).
In Uruguay, the incidence of tuberculosis Mycobacterium is low and data on pulmonary tuberculosis cases but caused by non-tuberculosis Mycobacterium (MNT) -normally saprophytes- is lacking.
From a therapeutic point of view, diagnosis based on an accurate identification of Mycobacterium infectant may be significant since treatment and management differ according to the strain found.
Two DNA molecular markers were chosen in our laboratory to diagnose Mycobacterium through genotype identification: IS6110 insertion element and ribosomal DNA sequences 16s (DNAr 16s) to determine specific identity within Mycobacterium.
Once selected molecular techniques were updated, we undertook a retrospective study of 80 isolates identified as Mycobacterium by phenotype methods. Most of the isolates (75/80) were tuberculosis Mycobacterium strains. The remained five were identified as MNT strains, of which three caused pulmonary infections.
References
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2) Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC (Jr). Color Atlas and textbook of diagnostic microbiology. 5a ed. Philadelphia: Lippincott-Williams & Wilkins, 1997: 893-952.
3) Brooks RW, Parker BC, Gruft H, Falkinham JO 3rd. Epidemiology of infection by nontuberculous mycobacteria. V. Numbers in eastern United States soils and correlation with soil characteristics. Am Rev Respir Dis 1983; 130(4): 630-3.
4) Springer B, Stockman L, Teschner K, Roberts GD, Bottger EC. Two-laboratory study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 1996; 34(2): 296-303.
5) Hale YM, Pfyffer GE, Salfinger M. Laboratory diagnosis of mycobacterial infections: new tools and lessons learned. Clin Infect Dis 2001; 33(6): 834-46.
6) Eisenach KD, Cave MD, Crawford JT. PCR detection of Mycobacterium tuberculosis. In: Persing DH, Smith TF, Tenover FC, WhiteTJ (eds.). Diagnostic molecular microbiology: principles and aplications. Mayo Foundation. Rochester, MN 55905. Washington: American Society for Microbiology, 1993: 191-6.
7) Clarridge JE 3rd, Shawar RM, Shinnick TM, Plikaytis BB. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine micobacte-riology laboratory. J Clin Microbiol 1993; 31(8): 2049-56.
8) Kirschner P, Meier A, Böttger E. Genotypic identification and detection of Mycobacteria: Facing novel and uncultured pathogens. In: Persing DH, Smith TF, Tenover FC, WhiteTJ (eds.) Diagnostic molecular microbiology: principles and aplications. Mayo Foundation. Rochester, MN 55905. Washington: American Society for Microbiology, 1993: 173-89.
9) Cave MD, Eisenach KD, McDermott PF, Bates JH, Crawford JT. IS6110: conservation of sequence in the Mycobacterium tuberculosis complex and its utilization in DNA fingerprinting. Mol Cell Probes 1991; 5(1): 73-80.
10) Nolte FS, Metchock B, McGowan JE Jr, Edwards A, Okwumabua O, Thurmond C, et al. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction an DNA hibridization. J Clin Microbiol 1993; 31(7): 1777-82.
11) Goldenberger D, Künzli A, Vogt P, Zbinden R, Altwegg M. Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing. J Clin Microbiol 1997; 35(11): 2733-9.
12) Relman D. Universal Bacterial 16S rDNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ (eds.) Diagnostic molecular microbiology: principles and aplications. Mayo Foundation. Rochester, MN 55905. Washington. American Society for Microbiology, 1993: 489-95.
13) Patel JB, Leonard DG, Pan X, Musser JM, Berman RE, Nachamkin I. Sequence-Based identification of Mycobacterium species using the MicroSeq 500 16s rDNA bacterial identification system. J Clin Microbiol 2000; 38(1): 246-51.
14) Gillespie SH, McHugh TD, Newport LE. Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex. J Clin Microbiol 1997; 35(3): 799-801.
15) Cave MD, Eisenach KD, Templeton G, Salfinger M, Mazurek G, Bates JH, et al. Stability of DNA fingerprint pattern produced with IS6110 in strains of Mycobacterium tuberculosis. J Clin Microbiol 1994; 32(1): 262-6.
16) Sola C, Horgen L, Goh KS, Rastogi N. Molecular fingerprinting of Mycobacterium tuberculosis on a Caribbean island with IS6110 and DRr probes. J Clin Microbiol 1997; 35(4): 843-6.
17) Doolittle WF. Phylogenetic classification and the universal tree. Science 1999; 284(5423): 2124-9.
18) Higgins DG, Bleasby AJ, Fuchs R, Clustal V. 1:07: improved software for multiple sequence alignment. Comput Appl Biosci 1997; 8(2): 189-91.
19) Swofford DL. PAUP*: Phylogenetic analysis using parsimony. (*and other methods). Version 4.0b2. Sunderland, Massachusetts: Sinauer Associates, 1999.
20) Wallace RJ Jr, Cook JL, Glassroth J, Griffith DE, Olivier KN, Gordin F. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. American Thoracic Society. Official statement approved by the board of directors. Am J Respir Crit Care Med 1997; 156(2 Pt 2): S1-25.
21) Se Thoe SY, Tay L, Sng EH. Evaluation of Amplicor- and IS6110-PCR for direct detection of Mycobacterium tuberculosis complex in Singapore. Trop Med Int Health 1997; 2(11): 1095-101.
22) Brown BA, Wallace RJ Jr. Infectious due to nontuberculous mycobacteria In: Mandell GL, Bennett JE, Dolin R. Principles and practice of infectious diseases. 5ª ed. Philadelphia: Churchill Livingstone, 2000: 2630-6.
23) Metchock BG, Nolte FS, Wallace RJ Jr. Mycobacterium. In: Murray PR, Baron JO, Pfaller MA, Tenover FC, Yolken RH eds. Manual of clinical microbiology. 7th ed. Washington: American Society for Microbiology, 1999: 399-437.
24) Wallace RJ Jr, Swenson JM, Silcox V, Good RC, Tschen JA, Stone MS. Spectrum of disease due to rapidly growing mycobacteria. Rev Infect Dis 1983; 5(4): 657-79.
25) Bazerque E. Comisión honoraria para la lucha antituberculosa y enfermedades prevalentes. Informe trienio 1995-97. Montevideo: Ministerio de Salud Pública, 1997: 22 p.
26) Kochi A. The global tuberculosis situation and the new control strategy of the World Health Organization (WHO). Tubercle 1991; 72(1): 1-6.
27) Cohn DL, F Bustero, MC Raviglione. Drug resistance tuberculosis: review of the worldwide situation and the WHO/IUATDL Global Surveillance Project. Clin Infect Dis 1997; 24(suppl 1): S121-S130.
28) Raviglione MC, Snider DE Jr, Kochi A. Global epidemiology of tuberculosis. Morbidity and mortality of a worldwide epidemic. JAMA 1995; 273(3): 220-6.
29) Goodman RA, Foster KL, Trowbridge FL, Figueroa JP. Global disease elimination and erradication as public health strategies: Proceedings of a conference; 1996 Feb 23-25. Atlanta Georgia, EEUU. Bull WHO 1996; 76(suppl 2): 48.
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2002-12-31
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Cortinas MN, Fernández M, Valeta MI, Uriarte M del R, Mogdasy MC. Genotype characterization of 80 strains of Mycobacterium in Uruguay. Rev. Méd. Urug. [Internet]. 2002 Dec. 31 [cited 2024 Nov. 24];18(3):230-8. Available from: https://revista.rmu.org.uy/index.php/rmu/article/view/965
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